Creative Developments (Cosmetics) Limited

Active Ingredients 1997

 

Active Ingredients for Skin Care; Manufacturing Chemist 1997

 

Copyright John Woodruff

 

Over the last few years the main innovations in active ingredients for skin-care have been in the use of alpha hydroxy acids, ceramides and enhanced delivery systems. Sun protection has been to the fore and the use of inorganic oxides has stimulated many new formulations. Many natural materials have moved from folklore to proven efficacy[Ref 1]  and more compounds of natural origin are introduced than from any other category at conferences and symposia.

 

A review of the papers and posters contributed to the 19th IFSCC Congress [REF 2] reveal that UV protection and anti-inflammatory responses were considered of major importance. This may be due in part to the location of the Congress and the many papers and delegates from Japan and Korea. Skin lightening is currently of interest in both hemispheres; in the East all exposed areas are treated while in the West, products are more frequently designed for reducing local pigmentation.

 

The majority of ingredients for reducing skin pigmentation are tyrosinase inhibitors and a paper at the IFSCC Congress [REF 3] described their action and an in-vitro method for determining efficacy. As is usual with such tests the base line is provided by hydroquinone and the test material, an extract from Broussonetia kazinoki (paper mulberry) and trade named Kazinol F was shown to be more effective than kojic acid, ascorbic acid and hydroquinone. The anti-erythema and free radical scavenging activity of Kazinol F was also described and the material shown not to be a sensitiser nor a primary irritant. Another paper [REF 4] described the skin whitening effects of Polygonum cuspidatum (flowering bamboo) which inhibits tyrosinase synthesis and a third paper reported the results of screening one hundred plant extracts for tyrosinase inhibition [REf 5]. Morus alba and Glycyrrhiza glabra showed >50% inhibitory activity against mushroom tyrosinase at a concentration of 333mg/ ml. Other workers [REF 6] reported the effects of a liquorice extract on tyrosinase activity and as an anti-inflammatory and anti-allergen agent; concluding that the material was ideal as an ingredient for reducing pigmentation in cosmetic products for sensitive skin.

 

Another material shown to be effective [REF 7} is 2.5-dimethyl-4-hydroxy-3[2H]-furanone, (DMHF), an antioxidative compound from the reaction of L-cysteine/D-glucose. It was shown to be an efficient free radical scavenger and to have an inhibitory effect on the auto-oxidation of linolenic acid. In addition, DMHF appeared to prevent the cellular melanogenesis in cultured murine melanoma cells more effectively than kojic acid although it was not so effective as the latter for the inhibition of tyrosinase.

 

Arctostaphylos uva-ursi or bearberry is generating a lot of interest in skin whitening products. C.I.T.Sarl report that an extract of the plant has been shown to inhibit melanin production in human melanocytes by reducing tyrosinase activity and test results show it to be more effective than either kojic acid or L-ascorbic acid. Sederma have also investigated the skin whitening effect of bearberry; it has combined it with an extract from a mitracarp indigenous to West Africa and called it Etioline for which a synergistic effect in inhibiting tyrosinase activity is claimed.

 

Products to reduce cellulite continue to grow in popularity and traditionally such products have been based on seaweed and their derivatives so it is not surprising that ingredient suppliers are investigating these materials to find ways of improving their activity. Rhodysterone from Secma is a red seaweed extract with a lipolytic effect on adipose cells. The efficacy of Rhodysterone has been tested by both in-vivo and in-vitro methods and the suppliers claim an improvement in appearance, texture and suppleness of skin when applied to the thigh area of human volunteers. Exsymol have a number of materials based on organo-silicone compounds such as methylsilanol mannuronate (Algisium C) and a mixture of this with caffeine (Cafeisilane C) for slimming and anticellulite products. Exsymol also supply a theophyllin derivative, methylsilanol carboxymethyl theophylline alginate or Theophyllisilane C, and publish extensive literature describing the mechanism of cellulite and cellulite reduction with test protocols and results. Another company producing interesting organo-silicone ingredients is The Fanning Corporation. Dimethicone copolyol eicosanate is the result of complexing silicone with meadowfom seed oil triglycerides and by controlling the reaction three products with different solubilities are produced. The water-soluble version has been found to deeply penetrate the hair shaft with excellent results on hair conditioning. The oil-soluble version adds emolliency to skin care products and the water-dispersible version blooms and foams in water making it ideal for bath products.

 

Until recently many suppliers of botanical extracts have claimed an activity and then attributed the evidence to folklore which is less than adequate to prove efficacy as required by the 6th Amendment. Some companies are prepared to investigate the properties of such materials and publish properly validated test results. Thus a paper presented at the Active Ingredients Conference in Paris [REF 8] by Alban Muller described the development and testing of an anticululite ingredient, Slimming Phytomine Biocomplex which included caffeine from coffee, theophyllin from tea, asiaticosides from an Asian plant, Centella asiatica, and an extract of Aesculus hippocastanum or horsechestnut.[REF 9] Tests on female volunteers for eight weeks showed a significant improvement in skin texture, tone and 30% reported a reduction in the orange peel effect. Laboratoires Phybiotex of the Sederma group also produce botanically derived materials and substantiate claims by reporting the results of clinical studies. Phytotal SL complex contains extracts of Sambucus nigra (elderberry), Verbena officinalis (vervain) and Eugenia caryphyllata (clove tree) and was shown to reduce cellulite when applied in a cream at 5% concentration. The same company produces Phytotal FM for firming skin and improving tone and Phytotal VT for combating couperose and improving microcirculation with test results of clinical trials.

 

Sederma has recently introduced Cyclolipase which is claimed to control lipolysis of subcutaneous fat by the action of lipase, caffeine and AMP and has mixed an extract of Sambucus nigra (elderberry) with glyceryl polyacrylate and ethoxydiglycol to prepare a material trade-named Redulite that is claimed to attack the problem of retention of interstitial water by hypodermal adipose tissue. It needs to be used at from 15 - 20% to be effective and the mechanism is mainly due to osmotic pressure. Polyacrylate and methacrylate gels are also used as moisturisers and have been found effective as antimicrobial compounds so such ingredients are multifunctional. A paper presented at the S.C.S. Symposium on microbiological matters [REF 10] described the use of Osmocide, a polyacrylate gel, as a possible means of preparing preservative-free cosmetics or of greatly enhancing the activity of existing preservatives enabling lower levels to be used. [REF 11]

 

A totally different approach to treating cellulite was the subject of a paper given at the 19th IFSCC Congress. A cream containing barium-hexaferrite particles was claimed to produce a static magnetic field, the strength and depth of penetration of the magnetic field depending on the concentration of particles. [REF 12] Studies showed that the electromagnetic field could penetrate to a depth of from 0.1 to 0.5mm and there was a statistically positive increase in blood flow in the microcirculatory system. The paper describes the extensive studies necessary to prove slimming and anticellulite action of topically applied preparations and should stimulate many good ideas to those interested in the subject.

 

Anti-free radical activity is another term which is often seen and seldom quantified, especially under conditions of actual use such as in the final cosmetic composition. Ever since the free radical theory of cell damage and skin ageing was introduced in the late eighties botanical extract manufacturers have claimed an effect for various materials. In a paper presented at the Active Ingredients Conference in Paris by Thomas Herling [Ref 13] the skins susceptibility to oxidative damage and its own defensive and post-damage repair mechanisms were described. Herling then proposed an in-vitro method for determining the Radical Protection Factor (RPF) of a cosmetic formulation using Electron Paramagnetic Resonance Spectroscopy. The RPF depends on the test radical concentration and the concentration of the antioxidant system in the cosmetic formulation and is a value which describes the radical scavenging activity of the complete product. The same authors also describe a method of measuring the Antioxidative Factor (AOF) of the skin. It is an in-vivo method and measures the rate of decay of a test radical in the skin which is dependent on the nature and concentration of the test radical and the properties of the skin and Herling has found a correlation between RPF and AOF.

 

Enhanced delivery systems were described in Manufacturing Chemist, March 1996 and since then further advances have been made including the introduction of nanocrystalline materials from Nanophase Technologies Corporation. NTC has developed a vapour condensation process which, it is claimed, produces nanocrystalline particles which are spherical and have a narrow particle size distribution. The particles themselves may be prepared from titanium dioxide or zinc oxide and used as inorganic sunscreen agents or be iron oxide suitable for use in decorative cosmetics. Other metallic or ceramic materials may be used and the nanocrystals supplied in a variety of cosmetic oils or in aqueous dispersions.

 

REF 1. Press release on the Internet

 

REF 2    19th IFSCC Congress, Sydney, Australia, 1996.

 

REF 3    The Depigmentation effect of a new material extracted from paper mulberry and its comparison by three colorimetric instruments. Ha J.H, Jo N.S, Lee H.K, Kim J.I, Lee B.G, Park W.J, Pacific Corp., Korea

 

REF 4                Development of active cosmetic agents for skin whitening: inhibitory effects of flowering bamboo (Polygonum cuspidatum) on melanogenesis of B16 melanoma cells. K.Obayashi et al. Noevir Co. Ltd., Shiga Central Laboratory, Yokaich, Shiga, Japan

 

REF 5               Biological screening of 100 plant extracts for cosmetic use (II) Inhibitory activities of tyrosinase and DOPA autooxidation. Jeong Ha Kim1 et al.R & D Centre, Koreana Cosmetics Co.

 

REF 6               Application of liquorice extract and its derivatives to skin care products. Masato Tagawa et al, Nikko Chemicals Ltd.

 

REF 7 Anti-melanogenesis effect of 2,5-dimethyl-4-hydroxy-3 [2H]-furanone. Che-Ok Jeonet al. Kim Pacific Corp. & Speciality Chemical Research Institute, Korea.

 

REF 8 The Active Ingredients Conference, Paris 1996.

 

REF 9               Slimming Phytoamine Biocomplex; Muller.A.

 

REF 10 Society of Cosmetic Scientists (UK) Symposium 1996, Microbial contamination, determination and eradication.

 

REF 11 Physical methods for the preservation of cosmetic products; Dr. Karl Lintner, Sederma SA.

 

REF 12 A new direction in cosmetics: physics instead of chemistry. Effect of a static magnetic field on human skin; Zastrow L. et al. Benckiser Cosmetics, International Research Centre, Monaco.

 

Ref 13 Free radical scavenging activity of cosmetics; Herling T et al, Institute for Dermatological Research, Berlin.